Treponema pallidum specific antibody chemical light emitting detection kit and preparation method thereof

2014 
The invention discloses a treponema pallidum specific antibody chemical light emitting detection kit and a preparation method thereof, and relates to treponema pallidum. The kit comprises an outer package box, an alkaline phosphatase marked recombinant antigen bottle, a light emitting substrate bottle, a treponema pallidum specific antibody negative control bottle, a treponema pallidum specific antibody positive control bottle, a washing liquid bottle and a recombinant antigen coated micropore plate. The preparation method comprises the following steps: firstly, preparing reponema pallidum specific recombinant antigen and the recombinant antigen coated micropore plate, marking alkaline phosphatase of the recombinant antigen, further preparing a light emitting substrate, a washing liquid and a control group, and finally assembling the treponema pallidum specific antibody chemical light emitting detection kit. The treponema pallidum specific antibody chemical light emitting detection kit can be used for detecting syphilis specificity specific antibodies in clinical specimens, specific solid phase is adopted, immunity and light emitting reaction can be rapidly completed within a short time, the light emitting signals are greatly intensified, the sensitivity is improved, the detection time can be shortened, and the precision is improved.
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