Studies on the efficacy of inactivated propolis fowl cholera vaccines with mice instead of chickens

In order to develop a better method used as efficacy of inactivated propolis fowl cholera vaccines, this research used mice instead of chickens as laboratory animals. The results indicated when the weight of each (immuned) mouse was 15～18 g, the immune dose of vaccines was only 0.3 mL per mouse, the weight of each challanged mouse was 20～25 g after immunization of 10 days, and the challange dose was 5～9 CFU per mouse, mice could be used to efficacy of inactivated propolis fowl cholera vaccines instead of chickens. The qualification standard was that 8/10 (chickens) in immunized group were survival and 5/5 chickens in control group died. This method of efficacy took as less as 7 days with less examine cost, and be sensitive, specific, objective and convenient.