Safety,stability and immunogenicity of attenuated Salmonella typhimurium carrying MIC-5 gene of Eimeria stieda

The security,stability and immunogenicity trials of recombinant Salmonella typhimurium X4550 carrying rabbit's Eimeria stiedai MIC-5 gene expression plasmid asd-pBMIC-5-IL-15(X4550/asd-pBMIC-5-IL-15) was assayed in this study.The results showed that the 109 CFU inoculation dose was safe in the experimental rabbits.The recombinant plasmid existed stably in X4550 after 30 passages,which displayed a good genetic stability.After two immunizations in rabbits,the recombinant Salmonella typhimurium induced the specific antibodies against rabbit Eimeria stiedai and significantly improved the level of lymphocyte proliferation.The anti-coccidia index reached 164.4.The results indicated that the regulation DNA vaccine mediated by Salmonella had a good security,stability and immunogenicity against rabbit Eimeria stiedai.