Oocyte orientation selection method based on the minimum strain position in the penetration process

In this paper, we proposed an oocyte orientation selection method based on the minimum strain position in the penetration process in order to maximize the developmental potential of the operated oocytes. Considering the symmetry of an oocyte during the penetration process, we analyzed only the intracellular strain of the lower half of the oocyte (from 3 o’clock to 9 o’clock). Firstly, the strain distribution in the penetration process was calculated using the finite element analysis method. The influences of penetration force, zona pellucida thickness, oocyte radius, Young’s modulus of the cytoplasm, and zona pellucida on the strain distribution were studied. The simulation results showed that the strain values at the 4 o’clock and 9 o’clock areas were significantly smaller than those at the 5 o’clock, 6 o’clock, 7 o’clock, and 8 o’clock areas. Secondly, the experimental strain distribution during penetration was evaluated for the first time using an optical flow method. The experimental strain distributi...