Bacterial culture and antibiotics sensitivity of pathogens isolated from patients with biliary tract infection.

OBJECTIVE To analyze the distribution of pathogens and antibiotics sensitivity in patients with biliary tract infection,and to provide reference for reasonable application of antibiotics in clinics.METHODS Totally 162 bile samples were collected for bacterial cultures and drug sensitivity test.RESULTS Of the total 162 bile samples,pathogens were found in 56 samples,the positive rate was 34.57%,of which,2 were with mixed infection.Totally 58 pathogens were found,of which 35 strains were Escherichia coli(60.34%),6 ESBLs-producing E.coli strains(17.14%),5 strains Klebsiella pneumoniae strains(8.62%) and 4 strains Enterococcus faecium strains(6.90%).Thirty-two samples were detected anaerobic bacteria also,of which there was no anaerobic.Drug sensitivity results showed that the susceptibility rates of Gram-negative bacteria to imipenem,meropenem and amikacin were over 90.00%,to enzyme inhibitors and netilmicin over 80.00 %;but resistant to penicillins,cephalosporins and quinolones.The susceptibility rates of Gram-positive bacteria to teicoplanin,vancomycin,linezolid and minocycline were 100.00%,to quinutristin/dalfopristin,and rifampicin were 88.89%,but to macrolides,nitrofurantoin,aminoglycosides and quinolones were low.CONCLUSION E.coli is the main pathogen in biliary tract infection.Rational application of antibiotics in clinics should be made on the basis of the results of drug sensitivity test.