Влияние органических веществ и хлористоводородной кислоты на строение гидратного покрова панкреатина

The effect of organic compounds and hydrochloric acid on the state of water, adsorbed by pancreatin powder with fixed amount (50 mg/g) of residual water was studied using the method of low-temperature 1H NMR spectroscopy. It is shown that the water, adsorbed on solid particles of pancreatin exists in the form of clusters with various degree of association and binding strength. Chloroform stabilizes adsorbed water in clusters with chemical shifts δН = 3.5 and 4.5 ppm and average radius 15 and 6 nm, respectively. H2O-HCl clusters (δН = 8 ppm) appears in the presence of HCl . Water from these clusters freezes at a relatively high temperature in crystals with average radius 2 or 20 nm. In CDCl3-CD3CN or CDCl3-DMSO mixtures  the presence of polar compounds has small effect on the chemical shift of the adsorbed water. It shows poor solubility of these substances in clusters (domains) of strongly associated water.