Variation in phytoplankton pigment composition in relation to mixing conditions in temperate South-Central Chilean lakes

Abstract Thermal lake properties are sensitive to changes in windiness and precipitation, and affect the physical and chemical properties of the water column, which in turn control phytoplankton dynamics and primary production. We assessed the use of phytoplankton pigment profiling as a potential indicator of stratification conditions in temperate lakes in South-Central Chile. Spring and early summer phytoplankton pigment profiles and the physical and chemical limnology were analyzed in 43 lakes ranging in size, depth, altitude and catchment characteristics. Eleven lakes were sampled during both seasons. Variation in pigment composition between lakes was primarily related to stratification conditions and mixed layer light availability at the time of sampling. The dinoflagellate marker pigment peridinin was more abundant in more deeply mixed lakes with a lower mean irradiance, while chlorophyte pigments (chlorophyll b, lutein) tended to be higher in shallow (high-light) epilimnia. Diatom and chrysophyte pigments (fucoxanthin) dominated under less thermally stable and more variable light conditions. Cyanobacteria pigments (zeaxanthin), probably derived from picocyanobacteria, were relatively more abundant in very transparent, low productive lakes. Lakes in close vicinity of active volcanoes were enriched in silica and PO4-P concentrations and characterised by elevated chlorophyte marker pigments. Within strongly stratified lakes, in which the euphotic zone extended in the hypolimnion, cryptophyte pigments (alloxanthin) characterized the deep chlorophyll maxima while the epilimnion was consistently enriched with the photoprotective xanthophyll-cycle pigment violaxanthin. We conclude that major algal groups, represented by pigment biomarkers, are largely driven by changes in lake water column stratification and related mixed layer light availability as well as nutrient concentrations in temperate Chilean freshwater lakes.