[Inhibition of invasiveness and expression of heparanase-mRNA in human malignant melanoma cell line A375 by matrine].

Objective: To investigate the effect of matrine on the invasiveness and expression of heparanase-mRNA in human malignant melanoma cell line A375.Methods: The A375 cells were treated by matrine in different concentration.The total RNAs were extracted from the cells 48 hours after treatment and then semi-quantitative RT-PCR were performed to evaluate the heparanase-mRNA expression levels.Effect of matrine on adhesion of treated A375 cells was tested by cell-Matrigel adhesion assay.The invasiveness of treated A375 cells was measured by Matrigel invasion assay.Results: The hepanase-mRNA expression,adhesion and invasiveness of A375 cells treated with matrine of different final concentrations significantly decreased compared with that of the controls(p0.01).Besides,the inhibitory effects were signifcantly different when the cells treated with matrine of different concentrations(P0.01).Conclusion: By down-regulating the expression of heparanase-mRNA,matrine has a significant inhibitory effect on the adhesion and invasiveness of human malignant melanoma cell line in a dosedependent manner.