Anti-Proliferative Effects of Selected Dietary Polyphenols on Human Colon Carcinoma Cell Line, T84

Polyphenols are naturally occurring compounds in plants which are typically involved in defense against ultraviolet radiation or pathogens. Previous studies have shown that various polyphenols have anti-proliferative properties in a variety of human cancers. The purpose of this study is to determine the effect of Quercetin (QC), Epigallocatechin-3-gallate (EGCG), Catechin and Resveratrol (RVL) on cell death and apoptosis in human colon carcinoma, T84, cells. Cell death and apoptosis was measured by using Annexin V: FITC Apoptosis Detection Kit I and a flow cytometry. Short term (1 hour) and long term (overnight; O/N) effects were observed. Cells were grown in 6 well plates, serum starved O/N and treated with different concentrations of polyphenols for varying time. Linear Sweep Voltammetry (LSV) was used to electrochemically characterize the antioxidant capacity and to quantify active RVL and QC (the polyphenols acting as reducing agents) for the time frame of the study. While EGCG and Catechin did not ha...