Live Cell Imaging With Spatial Light Modulator-based Optical Sectioning Structured Illumination Microscopy

Structured illumination microscopy (SIM) is a method in fluorescence microscopy which works by acquiring a set of images using widefield detection. Each image in the set is made with a different position of an illumination mask, but with no mask in the detection path [1]. Subsequent image processing is used to produce an optically sectioned image (OS-SIM) [2 4], or an image with resolution beyond the diffraction limit (super-resolution SIM or SR-SIM) [5,6].