In vitro maturation of sheep prepubertal oocytes

The aim of the study was to determine concentration of the Estrus sheep serum (ESS) for sheep in vitro oocyte maturation. Sheep ovaries were collected from a local abattoir and transported within 1 h to the laboratory in a warm saline solution (30 – 35 oC), Cumulus-oocyte complexes (COC’s) were obtained by aspirating of follicles, washed in TCM-199 modification with 50 g/ml gentamycin, The COC’s were randomly divided into Three groups. Treatment I (n = 100) COC’s were fresh and cultured in TCM-199 medium with 10% ESS + Estrdio 17β + 10 µg/ml FSH+10µg/ml LH. Treatment II (n = 100) COC’s were washed five times and cultured in TCM-199 medium supplemented with Estrdio 17β + 10 µg/ml FSH+10µg/ml LH. Treatment III (n = 100) COC’s were washed five times and cultured in TCM-199 medium and gentamycin treated as control. the evidence of different stages of maturation. Significantly higher (p