輸血により抗Dを産生したと考えられる partial D の1症例

A case of partial D patient who formed anti-D by blood transfusion was reported.The patient was a 54-year-old Japanese man suffering from cerebral infarction. His blood group was B, Rho (D) positive and no irregular alloantibodies were detected in pretransfusion screening and crossmaching tests. He received 3 units of ABO-compatible Rho (D) positive concentrated red cells in treatment of anemia due to gastritis on May. 25-27, 1988.Anti-D was found in his serum on Nov. 16, 1989 when he was again hospitalized with sequelae of cerebral infarction. His red cells were shown to react neither with 2 samples of monoclonal anti-D (OSK3 and OSK-1, both of which were prepared by Osaka Red Cross Blood Center) nor 2 samples of commercially purchased monoclonal anti-D. But they did react with 4 samples of polyclonal anti-D by both anti-globulin test and indirect immunof luoresence flow cytometry.His anti-D did not react with D category VI red cells, but reacted with D category IVb and Va cells. The results obtained indicated that he has D category VI red cells and formed anti-D by the transfusion of D-positive red cells.