Determination of amino acids in Flos Lonicerae by HPLC coupled with pre-column derivatization.

Objective:To establish a method for determination of amino acids Flos Lonicerae and provide experimental basis for the physical basis of properties. Methods:The total proteins were separated,after that,hydrolyzed the total proteins,followed by pro-column derivation with Phenylisothiocyanate( PITC) and determined peak area of every amino acid by RP-HPLC method,at last calculated the cotent of every amino acid. Results:Using HPLC every amino acid can be separated well. Conclusion:This meth-od is a good way in the determination of amino acid content.