Noninvasive In Situ Ratiometric Imaging of Biometals Based on Self-Assembled Peptide Nanoribbon.
2020
Development of probes
for accurate sensing and imaging of biometals in situ is still a growing
interest owing to their crucial roles in cellular metabolism, neurotransmission,
and apoptosis. Among them, Zn2+ and Cu2+ are
two important cooperative biometals closely related to Alzheimer’s
disease (AD). Herein, we developed a multifunctional probe based on
self-assembling peptide nanoribbon for ratiometric sensing of Zn2+, Cu2+, or Zn2+ and Cu2+ simultaneously. Uniform peptide nanoribbon (AQZ@NR) was rationally
designed by coassembling a Zn2+-specific ligand AQZ-modified
peptide (AQZKL-7) with peptide KL-7. The nanoribbon further combined
with Cu2+-sensitive near-infrared quantum dots (NIR QDs)
and Alexa Fluor 633 as an inner reference molecule, which was endowed
with the capability for ratiometric Zn2+ and Cu2+ imaging at the same time. The peptide-based probe exhibited good
specificity to Zn2+ and Cu2+ without interference
from other ions. Importantly, the nanoprobe was successfully applied
for noninvasive Zn2+ and Cu2+ monitoring in
both living cells and zebrafish via multicolor fluorescence imaging.
This gives insights into the dynamic Zn2+ and Cu2+ distribution in an intracellular and in vivo mode, as well as understanding
the neurotoxicity of high concentration of Zn2+ and Cu2+. Therefore, the self-assembled nanoprobe shows great promise
in multiplexed detection of many other biometals and biomolecules,
which will benefit the diagnosis and treatment of AD in clinical applications.
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