The Influence of Estrogen Treatment on Induction of Mammary Carcinoma in Rats by Single-Dose Gamma Irradiation at Different Ages

The effect of age at exposure on induction of mammary carcinoma was studied in female rats of the inbred WAG/Rij strain that were treated with estrogen. Groups of 40 animals were exposed to a single total-body dose of 1 or 2 Gy of137 Cs γ radiation at age 8, 10, 12, 15, 22, 36 or 64 weeks. Hormone levels in the animals were increased by implantation of a pellet containing Estradiol-17β 2 weeks prior to irradiation. Animals were killed when moribund. All mammary tumors were resected and classified histologically as carcinoma or fibroadenoma. The age-specific incidence of mammary carcinoma was compared with that in control groups of unirradiated estrogen-treated rats using lifetime statistical analysis with both parametric and nonparametric methods. The excess normalized risk of carcinoma was 7.7 for both 1 and 2 Gy in the age groups 8-15 weeks, with no significant differences between the age groups. However, in the age groups 22-64 weeks, the excess normalized risk decreased with increasing age at exposure...