[Influence of Erigeron breviscapus on the expression of collagen type I , MMP1 and TIMP1 of MRC-5 cells under hypoxia].

Objective Study the influence of erigeron breviscapus on collagen typeⅠ,matrix metalloproteinase1(MMP1) and tissue inhibitor of metalloproteinase1(TIMP1) in human embryo lung fibroblast MRC-5 under hypoxia.Methods MRC-5 cultured in vitro were individed into 4 groups,including normoxia control,hypoxia,hypoxia and staurosporine(SP),hypoxia and erigeron breviscapus.The expression of hypoxia-inducible factor-1α(HIF-1α),pro-col1α1 and phospho-smad2(p-smad2) were evaluated by Western blot.The level of collagen typeⅠ,MMP1 and TIMP1 in supernatant fluid were detected by ELISA.Results Expressed pro-col1α1,p-smad2 in MRC-5 cells under nomoxia were detected,but not HIF-1α.A low level of collagen typeⅠ,MMP1 and TIMP1 was also detected in the cell supernatant.In nomoxia secreted pro-col1α1,p-smad2,collagen typeⅠ,MMP1 and TIMP1,hardly secreted HIF-1α.Under hypoxia,the expression of all of them was increased when compared with those of under nomoxia condition(P0.05).MMP1 expression was significantly further up-regluted by pre-cubation with SP in hypoxia,however collagen typeⅠ,TIMP1 in supernatant fluid and pro-col1α1,p-smad2 in cells were inhibited(P0.05,vs.hypoxia group).Treated with Erigeron breviscapus(12.5 μg/mL,50 μg/mL) under hypoxia condition,significantly reduced expression of pro-col1α1,p-smad2,HIF-1α in MRC-5 cells and collagen typeⅠ,MMP1,TIMP1 in supernatant fluid were observed(P0.05,vs.hypoxia).Conclusion Erigeron breviscapus can decrase the expression of HIF-1α of MRC-5 cells under hypoxia condition by inhibit the expression of collagen typeⅠ and TIMP1 partly through the Smad2 signaling pathways.