Yield optimization in the kinetically controlled enzymic peptide synthesis

Abstract The yield and its time-dependence in acylenzyme mechanism-based enzymic peptide synthesis are controlled by the proteinase kinetic specificity. The maximum yield is limited by a non-equilibrium constant K max . Both K max and the time, t max , taken to attain the maximum yield, are directly related to the enzyme kinetic parameters. These relationships allow kinetic determination of yield optimization in kinetically controlled enzymic peptide synthesis.