Expression and significance of human triggering receptor on myeloid cells-1 in acute cholangitis

Objective To investigate the expression of human triggering receptor on myeloid cells-1 (TREM-1) and its regularity or clinical significance in patients with acute cholangitis,and discuss the relationship of TREM-I ,TNF-α and CRP in acute cholangitis. Methods Peripheral blood of 24 patients with ACST,34 patients with AC and 60 health adults were collected respectively. TREM-1 mRNA and protein was detected by semi-quantitative RT-PCR and immunocytochemistry respectively. Results The values of TREM-1/β-actin in control group was 0. 458 ± 0. 043, while those in AC and ACST group were 0.809 ± 0.044 and 0.981 ± 0.089 before therapy, and 0.785 ± 0. 077 ,0. 954 ± 0. 072 at 24 h, 0. 565 ± 0. 009 , 0. 719 ± 0.047 at 48 h, respectively. The level of TREM-1 express in ACST was higher than in AC (P ＜ 0.05). Immunocytochemistry revealed the results similar to those of RT-PCR. TREM-I expression was closely related with TNF-α, CRP expression ( r1 = 0. 719, r2 = 0. 701, P ＜ 0.01 ). Conclusion Expression of human TREM-1 was up-regulated obviously in patients with AC and ACST in the earlystage,indicating that TREM-1 might play an important role in the development of acute eholangitis. Key words: Acute cholangitis;  Triggering receptor expressed on myeloid cells-1;  TNF-α