Quantitative estimation of steroidal drug solasodine in wild species of Solanum by reversed-phase HPLC method

An improved reversed phase HPLC method for the quantification of Solasodine, an alternative precursor for the commercial production of steroid drug. The comparative analysis of solasodine in eight wild species of the genus Solanum was achieved via UFLC, SHIMADZU equipped with 254 nm. The 30 μL of filtered methanolic extracts was injected into Supelco reverse phased C18 analytical column at a flow rate of 1 mL/min at 30◦C and the effluent was monitored by UV detection at 205 nm. The solvent system was linear gradient of water and methanol from 40 to 70% for 25 min. Linear calibration curve was obtained between the amount of solasodine injected and the area under the peak over the concentration range of 0.1-1.0 mg mL-1 of solasodine with a correlation coefficient of 0.9999. The limit of detection (LOD) was found to be 0.012 μg mL-1. The limit of quantification (LOQ) value was found to be 0.035 μg mL-1.The method allows quantification of solasodine in plant tissue samples of S. diphyllum, S. incanum, S. nigrum, S. sisymbrifolium, S. surattense, S. torvum, S. verbascifolium and S. vellosum. The maximum concentration of solasodine found in S. sisymbrifolium 0.494 mg gm-1 and minimum in S. incanum 0.341mg gm-1.