EV71 3C protease action target point detection kit and EV71 3C protease action target point detection method

The present invention discloses an EV71 3C protease action target point detection kit, which comprises 3C protein, a polypeptide substrate and an enzymolysis reaction buffer solution, wherein the sequence of the 3C protein is represented by SEQ ID NO.1, and the polypeptide substrate is Dabcyl-EALFQGPPKFE-Edans. The present invention further discloses an EV71 3C protease action target point detection method, which comprises: adding a compound to be detected and 3C protein to an enzymolysis reaction buffer solution, adding a polypeptide substrate, uniformly mixing, and detecting the fluorescence intensity at a wavelength of 490 nm under 336 nm wavelength excitation, wherein it is indicated that the compound can inhibit the 3C protein if the fluorescence can not be detected, and it is indicated that the compound can not inhibit the 3C protein if the fluorescence can be detected. According to the present invention, with the detection kit and the detection method, the anti-EV71 ability of the compound to be detected can be effectively detected; and the method is simple and easy to perform, and the specificity and the sensitivity are high.