Establishment, characterization and chemosensitivity of two human glioma derived cell lines

Two continuous human glioma derived cell lines, LI and DF, were established in our laboratory. Both cell lines showed cytological features andin vitro behavior similar to those of the respective original neoplasms. These two lines were characterized for their main biological properties includingin vitro andin vivo growth rate, clonogenic ability and tumorigenicity in nude mice. The plating efficiencies were generally high both during exponential and stationary growth phases and a high tumorigenicity was observed. All injected nude mice developed tumors. The two lines were tested for chemosensitivity to 1,3-bis(2-chloroethyl)-1-1nitrosourea (BCNU) and cis-Diamminedichloroplatinum II (DDP).