[Antiviral activity of 3-hydroxyphthalic anhydride-modified ovalbumin against herpes simplex virus 2 in vitro].

Objective To investigate the antiviral activity of 3-hydroxyphthalic anhydride-modified ovalbumin(HP-OVA) against herpes simplex virus 2(HSV-2) in vitro.Methods By chemical modification,ovalbumin(OVA) was treated with 3-hydroxyphthalic anhydride(HP) to prepare HP-OVA.The anti-HSV-2 activity against HSV-2 333 virus in vitro and the cytotoxicity of HP-OVA in African green monkey kidney cells(Vero cells) were detected by MTT colorimetric assay.The inhibitory effects of HP-OVA on 17 strains of vaginal lactobacilli were observed by microscopy.Results Anhydride-modified ovalbumin significantly inhibited the infection by HSV-2 with an IC 50 of 23.56±8.33 μg/ ml.HP-OVA showed only low cytotoxicity to the host cells with a CC 50 over 1 mg/ml.HP-OVA did not produce significant inhibitory effect on the 17 strains of vaginal lactobacilli(MIC1 mg/ml).Conclusion Anhydride-modified protein HP-OVA exhibits potent anti-HSV-2 activity in vitro and can be a good microbicide candidate for prevention of sexually transmitted diseases.