In vitro anthelminthic and anti-inflammatory activity of ethanolic extract of Randia uliginosa DC Leaf

Ethanolic extract of Randia uliginosa DC Leaf (EERUL) was assessed for its anthelmintic and anti inflammatory activity by in vitro methods. Anthelminthic activity was performed on adult Indian earthworms, Pheretima posthuma due to its anatomical and physiological resemblance with that of intestinal round worm parasite of human beings, the bioassay involved determination of time of paralysis and time of death of the worms. Albendazole was used as standard reference. EERUL showed significant activity at the concentration of 100mg/ml with 44.50 and 58 minutes for paralysis and death respectively. In vitro anti inflammatory activity was evaluated using albumin denaturation assay, human red blood cell membrane stabilization assay and proteinase inhibitory activity at different concentrations. Diclofenac Sodium was used as a standard reference drug. The extract showed in vitro anti inflammatory activity by inhibiting the heat induced albumin denaturation, the percentage of inhibition was found to be 48- 85% EERUL and for the standard it was 47- 87% at the concentration range of 100 to 600 µg/ml. The EERUL and the standard showed maximum human red blood cells membrane stabilization of 67% and 78% at the concentration of 600 µg/ml respectively. Proteinase activity was also significantly inhibited by the EERUL it exhibited significant antiproteinase activity. It showed maximum inhibition of 66% at 600 g/ml and that of standard was found to be 73% at the same concentration. From the results, it can be concluded that the anthelminthic activity and anti inflammatory activity of EERUL may be due to the presences of secondary metabolites in the extract