CD138-resistant monoclonal antibody variable region sequence as well as preparation method and application thereof
2013
The invention discloses a CD138-resistant monoclonal antibody variable region sequence. The CD138-resistant monoclonal antibody variable region sequence comprises six nucleotide sequences, wherein the nucleotide sequences are 58208(1)-VH, 58208(1)-VL, 58208(2)-VH, 58208(2)-VL, 59166-VH and 59166-VL. Polypeptide synthesized by CD138 proteins and U266 cells are used as antigens; after mice are immunized, the mice of which the corresponding antibody expression is positive are detected; after splenocyte is separated; the splenocyte and myeloma cell are fused; after culture in HAT culture medium, an antibody expression positive clone is acquired by detection, and a positive hybridoma total ribonucleic acid (RNA) is extracted; because the mRNA in the total RNA is used as a template, cDNA of the corresponding antibody gene is obtained by inverse transcription; and the heavy chain variable region and light chain variable region of the corresponding antibody are acquired by using a specific primer polymerase chain reaction (PCR) so as to clone and sequence.
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