Generation and Characterization of Novel Monoclonal Antibodies Recognizing UBXD1

UBXD1 is a recently identified adaptor for p97, a highly abundant and conserved member of the AAA family of ATPase that plays pivotal roles in a multitude of cellular processes involving the ubiquitin-proteasome pathway. Very little is known about the biochemical, cellular, and molecular functions of UBXD1. Here we report the generation of two mouse monoclonal antibodies, 5C3–1 and 2F8–24, that recognize UBXD1 using Western blotting, immunoprecipitation, and immunofluorescence.