하고초(Spica prunellae) 추출물의 항비만 및 산화적 스트레스 억제활성

Spica prunellae has been used as a traditional medicine in Asian countries. In the present study, anti-obese and anti-oxidant activity of Prunellae spica ethanol extract (PSE) was investigated in 3T3-L1 and HepG2 cells. The 50 and100μg/mL of PSE treatment for 4 days (Day -2 to Day 2) dose dependently suppressed 3T3-L1 adipogenesis. Wefurther examined the protective activity of PSE against ethanol-induced cell death. While 800mM ethanol treatmentfor 24 h induced significant cell death, the PSE treatment significantly protected ethanol-induced cell death. In addi-tion, the anti-oxidative activities of PSE to reduce the oxidative stress induced by AAPH or Cu2+ ions, which areimportant ROS to release peroxyl and hydroxyl radicals, were observed. These results provide scientific evidencefor the development of functional foods using PSE which has anti-obese and antioxidant activities.