Method for identifying pathogenicity of sesame stem blight and blast pathogenic bacteria

2010 
The invention relates to a method for identifying pathogenicity of sesame stem blight and blast pathogenic bacteria. The method comprises the following steps of: (1) culturing bacterial strains for inoculation; (2) pretreating seeds, namely selecting healthy and full sesame seeds and sterilizing for later use; (3) inoculating, namely adding a sterilization culture medium into a culture bottle, laying down sterile filter paper, adding sterile water, removing bubbles, uniformly placing sesame sterile seeds along the edges of the filter paper, inoculating bacterium cakes in the center of the filter paper, and hermetically culturing in the dark; (4) investigating pathogenicity, namely dividing the pathogenic conditions into different pathogenic grades according to pathogenic degrees; and (5) evaluating the pathogenicity, wherein the pathogenic index is equal to Sigma (pathogenic grade*corresponding pathogenic bud number)/total bud number. The method is simple and convenient and practicable, the required equipment is few, the result is reliable, and the pathogenicity of different sesame stem blight pathogenic bacteria and blast pathogenic bacteria can be reflected more accurately.
    • Correction
    • Source
    • Cite
    • Save
    • Machine Reading By IdeaReader
    0
    References
    0
    Citations
    NaN
    KQI
    []