OCT based cross-sectional and three-dimensional dynamics imaging for visualization and quantification of tumor spheroid activity

We present a new no-invasive label-free OCT-based tissue dynamics/subcellular motion imaging method to visualize and quantify the tissue activity of tumor spheroid. Our method is based on the statistical analysis of the OCT intensity fluctuations of the rapidly acquired OCT signals. The analysis includes log intensity variance (LIV) and OCT correlation decay speed (OCDS). The presented methods have been utilized to visualize and quantify the necrotic activity of the human originated tumor spheroids along 20 hours as cross-sectional and 3-D tomography. This necrotic activity of the spheroid has been not only visualized as an image, but also quantification of the necrotic cell ratio in the spheroid region has been presented.