Antibacterial Activity of Brewer's Spent Grains Peptides to Staphylococcus aureus

Antibacterial ability of brewer's spent grains peptides was detected which were hydrolyzed in different hydrolysis time with four proteases. The results showed: Flavourzyme hydrolyze in 180 min has better antibacterial ability to Staphylococcus aureus. The hydrolyzate was separated with polyamide column chromatography, ion exchange column chromatography, respectively. Peptides were tested of antibacterial ability, and better antibacterial ability were collected. Molecular weight is about 1877.67 which is measured with gel column chromatography. The minimal inhibitory concentration to Staphylococcus aureus was 2%.