Adaptive optics line-scan OCT for high-speed imaging of retinal structure and function (Conference Presentation)

We developed a high-speed adaptive optics, line-scan spectral domain OCT and used it to characterize stimulus-induced optical path length changes in cones with high spatiotemporal resolution. We find that individual cone outer segments exhibit a biphasic light-induced response—a rapid axial shrinkage followed by a gradual increase in optical path length, both increasing in magnitude with the stimulus intensity. AO line-scan OCT thus offers high-speed volume acquisitions, high phase stability, sub-ms temporal resolution and cellular-scale spatial resolution, that together enable imaging retinal structure and function in health and disease.