Haemostatic property of Chromolaena odorata leaf extracts: in vitro and in vivo evaluation in wistar rats

This study was designed to investigate the effects of aqueous, ethanol and crude extracts of Chromolaena odorata leaf on haemostatic mechanism of wistar rats and its possible in vitro use in coagulation study. Fifty wistar rats of both sexes weighing between 140-180 g were sorted into 10 groups each fed via oral gavage once daily for 21 days. Sample collection was done by cardiac puncture. Bleeding and clotting times were performed using Duke’s and Ivy’s methods, respectively. The prothrombin time was performed using the Quick’s one stage method, while the partial thromboplastin time using kaolin was done using Macpherson and Hardity method using Giess diagnosis reagent. Significant results were observed in the bleeding and clotting times of the three extracts in a dose-dependent manner. The 300-mg/kg ethanol extract decreased the bleeding time more than the other two extracts. Only the ethanol 150 and 300 mg/mL showed in vitro activity. The study showed the in vivo haemostatic properties of Chromolaena odorata leaf extracts and its possible use in in vitro coagulation study.