Study on cerebrospinal fluid Differentiation of Mesenchymal Stem Cells into neuron in Vitro

Objective To investigate the feasibility of Cerebrospinal fluid induces human mesenchymal stem cells(MSCs) directed to differentiation into neural stem cells. Methods MSCs derived from adult bone marrow and new born cord blood were isolated and cultivated in vitro. MSCs were induced and differentiated by cerebrospinal fluid and growth factor,respectively.Morphological changes of induced cells were observed. The expression of cell surface antigens was detected by flow cytometer,immunocytochemistry and RT-PCR.Results MSCs were induced and differentiated into neuron like cells by cerebrospinal fluid and growth factor respectively. The induced cells expressed NSE,NF after induction. There were no significant differences between two groups in morphological changes. However,the growth cycle of neural stem cells and Neuron-like cells was shortened in the group induced by cerebrospinal fluid. Conclusion MSCs consecutively treated with cerebrospinal fluid and growth factor is induced to neuron-like cells,and the cell cycle and time induced by cerebrospinal fluid get shorter. Key words: Cerebrospinal fluid; MSCs; Neuron-like cells; Induced differentiation