Recent advances in gold nanoparticles-based biosensors for food safety detection.

Abstract Food safety issue remains a challenge worldwide. Common substances in food can pose a great threat to human health including but not limited to food borne-pathogens, heavy metals, mycotoxins, pesticides, herbicides, veterinary drugs, allergens and illegal additives. To develop rapid, low-cost, portable and on-site detection methods of those contaminants and allergens to ensure food safety, gold nanoparticles (AuNPs) of versatile shapes and morphologies such as nanorods, nanoclusters, nanoflowers, nanostars, nanocages, nanobipyramids and nanowires have been employed as probes because they possess extraordinary properties that can be used to design biosensors enabling detecting various contaminants and allergens. By means of surface modification, AuNPs can directly or indirectly sense specific targets based on different mechanisms, such as hydrogen bonds, nucleic acid hybridization, aptamer-target binding, antigen-antibody recognition, enzyme inhibition, and enzyme-mimicking activity. AuNPs can induce a distinct color change from red to blue when they transform from a monodispersed state to an aggregated state in liquid solution, which can be observed by naked eyes. If Raman molecules are functionalized on AuNPs, their aggregation will alter the interparticle distance and induce the surface-enhanced Raman scattering that can be employed for highly sensitive detection. Ultra-small AuNPs such as Au nanoclusters also feature in fluorescence that enable a fluorescent readout. The formats of AuNPs for food safety detection in real world range broadly including but not limited to films, fibers, liquid solutions, tapes, chips and lateral flow strips. In this review, recent applications of AuNPs-based biosensors for food safety detection will be discussed, mainly in the aspect of different contaminants and allergens encountered in food samples.