The evaluation of synergistic effect of Hippophae rhamnoides and vitamin E on growth performance and oxidative stress at Oreochromis niloticus-Linnaeus, 1758.

The aim of this research is to evaluate the influence of sea buckthorn ( Hippophae rhamnoides ) and vitamin E on growth performance indicators and oxidative stress at Nile tilapia juvenile, reared in a recirculating aquaculture system. The experiment was conducted six weeks, in triplicate. The experimental variants were: V1 – control, V2 – 1% sea buckthorn / kg feed, V3 – 500mg vitamin E / kg feed and V4 – 1% sea buckthorn supplemented with 500 mg vitamin E / kg feed. During the experiment was performed an intermediary biometric measurement. Oxidative stress analysis consisted in determination of lipid peroxidation (MDA-malondialdehide) and total antioxidant capacity (TAC) from liver, tissue and gut. Results showed a good evolution of GR, FCR and SGR, during the experiment, in V4 – in which feed was supplemented with sea buckthorn and vitamin E. Based on the results obtained in variant V4, in liver and tissue, the oxidative stress was reduced. Regarding MDA and TAC, between experimental variants, were registered significant differences (p<0.05) at the level of tissue and gut. In conclusion, the research shows that sea buckthorn (1%/kg feed) in combination with Vitamin E (500mg/kg feed) has a synergistic effect on growth performance indicators and oxidative stress, at Oreochromis niloticus juvenile.