Efficacy of the cat deafening method: Co-administration of ethacrynic acid and kanamycin

AbstractObjective: This study was designed to determine if hearing status monitoring during intravenous infusion of EA reduces individual variability and to evaluate the correlation between EA dose and Bwt.Materials and methods: Twenty-five cats with the mean age of 24 ± 3.7 weeks (range = 20.6–28.3) and a mean weight of 3.21 ± 0.84 kg (range = 1.9–5.1) were administered a subcutaneous injection of KM (300 mg/kg) followed by an intravenous infusion of EA (1 mg/min). Click-evoked auditory brainstem responses (ABRs) were recorded to monitor hearing during the infusion. When ABR thresholds exceeded a 90 dB sound pressure level, the infusion of EA was terminated. Histopathology forapex, middle, and base sections of the cochlea were examined after 6 months.Results: The dose of EA was optimized for deafening through simultaneous ABR measurements. Bwt was positively correlated with EA dose (mg) (p < 0.001, R2 = 0.548), which was different from a study previously reported. Cochlear histopathology assessments reve...