Cloning Nematode Acetylcholine Receptor Genes

Recombinant DNA technology has made it possible to clone receptors from many organisms by cross-hybridization or by the polymerase chain reaction. It may be difficult, though, to establish the functional importance of any clone obtained. We describe the cloning of nematode acetylcholine receptor genes by selection for resistance to levamisole, a scheme providing assurance that the clones obtained are functionally related.