Анализ возможностей лазерного флуоресцентного метода контроля состояния растений в стрессовых ситуациях, вызванных различными механическим повреждениями

Today one of the most promising lines to use a fluorescence analysis is to monitor the vegetation state. There are a large number of papers, which present the results of experimental studies of vegetation fluorescence spectra in normal and stressful situations. However, most works in the field concerned describe sensing the plants under stress caused by insufficient nutrients or pollutants in the soil. Currently, there are only a few experimental works, which study the fluorescence spectra of plants under stress caused by different mechanical damage of plants. The paper presents results of experimentally studied laser-induced fluorescence spectra of plants with different mechanical damage for the fluorescence excitation wavelength of 532 nm. To measure the fluorescence spectra was created laboratory setup. A solid state pulse YAG: frequency-doubled ND and diode-pumped solid-state laser has been used as a source of the fluorescence excitation. The highly sensitive array detector with an intensifier provided a record of the fluorescence spectrum. The paper studied the change in the laser-induced fluorescence spectra of plants when exposed to a diversity of mechanical damage such as cutting leaves of the plant; damage of the roots; trampled leaves. The paper conducts the experimental study of the fast growing and easy to keep plants (it shows data for the salad, as an example of crop plants). Experimental studies have shown that at the fluorescence excitation wavelength of 532 nm the effect of stress factor because of different mechanical damage may be evident as a changing form of the fluorescence spectrum. All experiments with plant stress have shown that at 680 and 740 nm wavelengths the ratio of fluorescence intensities significantly increases. This potentially allows us to use the laser fluorescence method to detect the stress situations of plants caused by mechanical damage.