Multiple Adhesive Layering Influence on Dentin Bonding and Permeability.

: The aim was to evaluate the effect of the number of layers on the microtensile bond strength (μTBS), permeability and nanoleakage of an etch-and-rinse adhesive (Adper Single Bond 2). Different numbers of layers (1, 2 and 4) were applied on dentin susbstrate. Specimens were obtained and tested for μTBS. Stick-shaped samples were analysed by scanning electron microscope, to observe silver nitrate penetration. One-way ANOVA and Tukey tests were applied (α=0.05) for μTBS values. The fracture mode was evaluated under a stereomicroscope and a scanning electron microscope (SEM). Groups with two and four layers of bond showed higher μTBS to dentin and they also showed lower adhesive permeability than ONE layer. The silver nitrate uptake occurred in the hybrid layer in practically all groups. Fracture occurred predominantly at the mixed interface. The authors recommend the application of more than one layer of Adper Single Bond 2, because a single layer showed lower μTBS and higher permeability values.