Segmental pycnotic reversion of facultative heterochromatin during the second male meiosis in tettigonioid species (Orthoptera)

The X chromosome invariably exhibits isopycnosis during the most condensed stages of male meiosis after conventional staining procedures in all studied species of Tettigonioidea (Orthoptera). However, differential negative staining of the X chromosome can be achieved during these stages by a silver impregnation method after extraction of chromosomal proteins with 2 × SSC. This differential response to silver may be indicative of a distinctive chromatin organization in the X chromosome with respect to that of the autosomes. Following 2 × SSC – silver impregnation treatment, pycnotic reversion of part of the X chromosome can be observed from the metaphase II stage on in tettigonioids. Pycnotic reversion is initiated from the telomeric region of the X chromosome in the subfamily Ephyppigerinae, while in the subfamily Pycnogastrinae pycnotic reversion occurs at the centromeric region of this chromosome. These two modes of segmental pycnotic reversion may indicate the onset of a reversion of the facultative he...