Preparation method of non-phospholipid nanodisc

The invention provides a preparation method of a non-phospholipid nanodisc, and is a characterized in that the preparation method includes the following steps: step one, in a membrane protein purification stage, adding a phospholipid-like detergent; step two, after completion of membrane protein purification, adding a membrane scaffold protein, mixing evenly with a uniform mixing device, and carrying out thermostatic incubation for a scheduled time; and step three, after purifying with molecular sieve and affinity chromatography, concentrating, and collecting the product. The method omits three steps in a traditional method of dissolving phospholipid in a detergent, mixing a membrane scaffold protein with phospholipid pre-dissolved in the detergent according to the proportion and removing the detergent with dialysis or a hydrophobic adsorbent. Therefore, the preparation steps of the non-phospholipid nanodisc are greatly simplified, and besides, the loss of membrane proteins is avoided.