1P-343: Heterologous expression and functional analysis of methanol and formaldehyde-assimilating enzymes in type I methanotrophs

Natural one-carbon metabolic pathways in native hosts such as methylotrophs have recently been reconstructed in non-native hosts mainly in methanol. Engineering strategy for methanol bioconversion was the implementation of NAD-dependent methanol dehydrogenase (MDH) and co-expressing two key enzymes in ribulose monophosphate cycle (RuMP) were hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi). MDH and several Hps-Phi gene clusters as well as artificial fusion forms from different type I methanotrophs were cloned and screened. As a results, in vitro activity of mdh and Hps-Phi were detected. The data provided new opportunities for reconstruction of methanol metabolic pathway from methanotrophs into non-native hosts.