Rapamycin inhibits the proliferation of prostate cancer cell line 22RV1 and activity of S6K1

Objective: To explore the effects of rapamycin on the proliferation of prostate cancer cell line 22RV1 and the activity of S6K1.Methods: Prostate cancer 22RV1 cells cultured in vitro were treated with rapamycin at the concentrations of 0,50,100,200 and 400 nmol/L.The inhibition rate of the cells' proliferation was detected by MTT,and the activity of S6K1 was determined by liquid scintillation counting.Results: Rapamycin significantly inhibited the proliferation of the prostate cancer 22RV1 cells and the activity of S6K1 in a dose-dependent manner,most obviously at 400 nmol/L(P0.01).Conclusion: Rapamycin can effectively suppress the proliferation of prostate cancer 22RV1 cells by regulating the expression of S6K1,the downstream protein of mammalian target of rapamycin(mTOR).