Epidemiological survey of Blastocystis hominis in Huainan City,Anhui Province,China

AIM: To provide scientific evidence for prevention andcontrolling of blastocystosis, the infection of Blastocystishomonis and to study its clinical significance in Huainan City,Anhui Province, China.METHODS: Blastocystis homonis in fresh stools taken from100 infants, 100 pupils, 100 middle school students and403 patients with diarrhea was smeared and detected withmethod of iodine staining and hematoxylin staining. Afterpreliminary direct microscopy, the shape and size ofBlastocystis homonis were observed with high power lens.The cellular immune function of the patients withblastocystosis was detected with biotin-streptavidin (BSA).RESULTS: The positive rates of Blastocystis homonis inflesh stools taken from the infants, pupils, middle schoolstudents and the patients with diarrhea, were 1.0 % (1/100),1.0 % (1/100), 0 % (0/100) and 5.96 % (24/403)respectively. Furthermore, the positive rates of Blastocystishomonis in the stool samples taken from the patients withmild diarrhea, intermediate diarrhea, severe diarrhea andobstinate diarrhea were 6.03 % (14/232), 2.25 % (2/89),0 % (0/17) and 12.31% (8/65) respectively. The positiverates of Blastocystis homonis in fresh stools of male andfemale patients with diarrhea were 7.52 % (17/226) and3.95 % (7/177) respectively, and those of patients in urbanand rural areas were 4.56 % (11/241) and 8.02 % (13/162)respectively. There was no significant difference betweenthem (P＞0.05). The positive rates of CD3+, CD4+, CD8+ inserum of Blastocystis homonis-positive and-negativeindividuals were 0.64±0.06, 0.44±0.06, 0.28±0.04 and0.60±0.05, 0.40±0.05 and 0.30±0.05 respectively, andthe ratio of CD4+/CD8+ of the two groups were 1.53±0.34and 1.27±0.22. There was significant difference betweenthe two groups (P＜0.05, P＜0.01).CONCLUSION: The prevalence of Blastocystis hominis asan enteric pathogen in human seems not to be associatedwith gender and living environment, and that Blastocystishominis is more common in stool samples of the patientswith diarrhea, especially with chron