Isolation, molecular identification and antibiogram of Pasteurella multocida in haemorrhagic septicaemia outbreak of buffaloes in West Bengal

Pasteurella multocida was isolated from clinically ill buffaloes in an outbreak of haemorrhagic septicaemia (HS). The isolates were identified by conventional culture along with molecular method of identification by polymerase chain reaction (PCR). An expected PCR amplicon of ∼460 bp and ∼620 bp products was observed using species specific PM-PCR, capsular type B specific PCR assays, respectively. In Kirby-Bauer disc diffusion method, the isolates were found to be sensitive to ofloxacin, ceftriaxone, cotrimoxazole, amikacin, gentamicin, intermediately sensitive to ciprofloxacin and resistant to tetracycline, ampicllin-salbactam, azithromycin, ceftazidime. PCR assays were found simple and quick diagnostic approach of haemorrhagic septicaemia and P. multocida capsular type B was found resistant to many antimicrobials.