Kororamide B, a Brominated Alkaloid from the Bryozoan Amathia tortuosa and Its Effects on Parkinson′s Disease Cells

NMR metabolite fingerprints of fractions from an extract of the bryozoan Amathia tortuosa allowed identification of three known (1–3) and evidence of a new tribrominated metabolite. The new compound, kororamide B (4), was purified through NMR-guided isolation and the structure assigned by mass and detailed NMR analysis. The absolute configuration of the new compound was established by comparison of the experimental and calculated electronic circular dichroism spectra. The compounds displayed effects on early endosomes when profiled on human olfactory neurosphere-derived cells (hONS) derived from a Parkinson's disease patient using a multidimensional phenotypic assay.