Withanolides from Aureliana fasciculata var. fasciculata

In the first phytochemical study of the Aureliana genus (Solanaceae), two new withanolides, 1 and 2, together with two known sterols, were isolated from the MeOH extract of the leaves of Aureliana fasciculata var. fasciculata. The structures were established as (4S,22R)-16α-acetoxy-5β,6β-epoxy-4β,17α-dihydroxy-1-oxowitha-2,24-dienolide (aurelianolide A) and (4S,22R)-16α-acetoxy-4β,17α-dihydroxy-1-oxowitha-2,5,24-trienolide (aurelianolide B). The new compounds possessed the unusual 16α,17α-dioxygenated group and were fully characterized by spectroscopic techniques, including 1H- and 13C-NMR (DEPT), as well as 2D-NMR (HMBC, HMQC, 1H,1H-COSY, NOESY) experiments, and HR-MS.