Hemicellulase-based engineering bacteria and realization method thereof
2014
The invention relates to hemicellulase-based engineering bacteria in the technical field of genetic engineering and a realization method thereof. The realization method comprises the following steps: firstly, by taking a vector pUC57-XL obtained by whole gene synthesis of streptomyces griseorubens genome DNA (deoxyribonucleic acid) as a template, performing PCR (polymerase chain reaction) amplification on the vector and a primer with an enzyme cutting site to obtain a nucleotide sequence XL for coding xylanase and a nucleotide sequence XO for coding xylosidase correspondingly and respectively; secondly, connecting the gene sequences obtained by amplification to a coexpression vector pETDuet-1 in sequence, and finally recombining a coexpression vector pETDuet-XL-XO; thirdly, converting the recombinant coexpression vector to an escherichia coli expression strain so as to obtain a transgenic coexpression strain. Therefore, a large quantity of xylanase and xylosidase can be expressed and synthesized in vitro by using genetic engineering means, and finally hemicellulose can be quickly degraded in situ.
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