Effects of glycyrrhiza flavonoid on lipid peroxidation and active oxygen radicals

The antiperoxidant activity of glycyrrhiza flavonoid (F G) was studied by using colorimetric estimation of lipid peroxide(MDA) formation. The scavenging effects of FG on O_2- and OH~- was investigated by using chemiluminescence method and spin trapping technique in different systems. The results were as follows. FG 2.8～25μg/ml effectively inhibited MDA formation induced by incubating mice liver homogenateat 37℃ for 1h; FG 0.265～26.5μtg/ml or 2.58～258μg/ml was shown to markedly scavenge O_2 in alkaline/DMSO or xanthine/xanthine oxidase systems, respectively.in a concentration-dependent manner. FG 144μg/ml or 258μg/ml also significantly scavenged the active oxygen radicals produced by PMN stimulation with PMA or OH~- produced in Fenton's reaction respectively. The results suggest that FG may be used as an antioxidant.