Effects of Dapperl expression on surviving-mediated cell apoptosis in gastric carcinoma

Objective To investigate the expressions of Dapper1 in gastric carcinoma and elucidate its relationship with survivin and its role in tumor cell apoptosis. Methods Dapperl mRNA was detected with RT-PCR using specimens from 30 cases of gastric carcinoma and the corresponding normal gastric mucosa. The pcDNA3. 1-Dprl plasmid was transfected into SGC-7901 cells with Lipofectamine^TM 2000. The effect of upregulation of Dprl on SGC7901 cell apoptosis was determined by flow cytometry. The downregulation of survivin,Dvl-2 and β-catenin protein expression were detected by Western blot analysis. Results Downregulation of Dprl gene expression was observed in 17 （57%） of 30 human gastric cancer and the downregulation was significantly correlated with the depth of invasion and the degree of differentiation （P 〈 0. 05）. Also, upregulation of Dprl mRNA and downregnlation of survivin mRNA were detected after transfecting pcDNA3. 1-Dprl plasmid in SGC7901 cells, which led to dowrrregnlation of survivin, Dv1-2, β-catenin protein and increase of the SGC7901 cell apoptosis rate from 2. 89% to 13.96%. Conclusion Downregulation of Dprl gene expression is common in human gastric carcinoma, and upregulation of Dprl results in significant inhibition of survivin expression which can induce apoptosis of SGC7901 cells. Key words: Stomach neoplasms ;  Apoptosis ;  Gene experssion;  Dapper1