Roseobactersp.zjut and application thereof in preparation of agaro-oligosaccharide
2010
The invention provides a novel agarase generating strain (roseobactersp.zjut) and application thereof in the preparation of agaro-oligosaccharide. The roseobactersp.zjut is preserved in the China Center for Type Culture Collection, the collection number is CCTCC No. M 209292, and the preservation date is December 3, 2009. The roseobactersp.zjut and the application thereof in the preparation of the agaro-oligosaccharide have the advantages that: (1) the roseobactersp.zjut of the invention has low requirements on nutrition and can be easily cultured; (2) agarase generated by the roseobactersp.zjut is an extracellular enzyme, separation and purification are convenient, and cell breakage is not required; (3) the roseobactersp.zjut has high agarase generating activity, and the activity of crude agarase liquid which is not separated and purified can reach 215.8U/ml under optimized conditions; (4) the agarase generated by the roseobactersp.zjut degrades agar under mild reaction conditions, and the reaction time is short; and (5) the agarase in the invention has high stability and basically has no enzyme activity loss (inactivation efficiency is less than or equal to 5 percent) after being stored in a refrigerator for 7 days at the temperature of less than 4 DEG C.
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